Appendix 1. Cochrane Gynaecology and Fertility Specialised Register search strategy

From inception to present

Procite platform

Keywords CONTAINS "IVF" or "ICSI" or "ET" or "intracytoplasmic sperm injection techniques" or "intracytoplasmic sperm injection" or "in‐vitro fertilisation " or "in vitro fertilization" or "Embryo Transfer" or "ovarian stimulation" or "ovarian stimulation controlled ovarian stimulation" or "ovulation induction" or "ovulation stimulation" or "superovulation" or "superovulation induction" or "ovarian hyperstimulation" or "poor responders" or "poor responder" or "poor prognostic patients" or "controlled ovarian hyperstimulation" or "controlled ovarian stimulation" or "COH" or Title CONTAINS "IVF" or "ICSI" or "ET" or "intracytoplasmic sperm injection techniques" or "intracytoplasmic sperm injection" or "in‐vitro fertilisation " or "in vitro fertilization" or "Embryo Transfer" or "ovarian stimulation" or "ovarian stimulation controlled ovarian stimulation" or "ovulation induction" or "ovulation stimulation" or "superovulation" or "superovulation induction" or "ovarian hyperstimulation"

AND

Keywords CONTAINS "uterine cavity injection" or "intrauterine flushing" or "Intrauterine injection" or "intrauterine instillation" or "flushing media" or "Flushing‐Outcome" or "stimulation of endometrium embryo transfer" or "endometrial preparation" or "endometrial priming" or "endometrial receptivity" or "endometrial stimulation" or "embryo culture supernatant" or Title CONTAINS "uterine cavity injection" or "intrauterine flushing" or "Intrauterine injection" or "intrauterine instillation" or "flushing media" or "Flushing‐Outcome" or "stimulation of endometrium embryo transfer" or "endometrial preparation" or "endometrial priming" or "endometrial receptivity" or "endometrial stimulation" or "embryo culture supernatant"

Appendix 2. CENTRAL (CRSO) search strategy

From inception to present

Web platform

#1 MESH DESCRIPTOR Embryo Transfer EXPLODE ALL TREES

#2 MESH DESCRIPTOR Fertilization in Vitro EXPLODE ALL TREES

#3 MESH DESCRIPTOR Sperm Injections, Intracytoplasmic EXPLODE ALL TREES

#4 embryo*: TI,AB,KY

#5 (vitro fertili?ation):TI,AB,KY

#6 ivf:TI,AB,KY

#7 icsi:TI,AB,KY

#8 (intracytoplasmic sperm injection*):TI,AB,KY

#9 blastocyst*:TI,AB,KY

#10 infertil* or subfertil*:TI,AB,KY

#11 assisted reproducti*:TI,AB,KY

#12 poor responder*:TI,AB,KY

#13 #1 OR #2 OR #3 OR #4 OR #5 OR #6 OR #7 OR #8 OR #9 OR #10 OR #11 OR #12

#14 (inject* adj5 culture*):TI,AB,KY

#15 (inject* adj5 medi*):TI,AB,KY

#16 (supernatant adj5 embryo*):TI,AB,KY

#17 (supernatant adj5 blastocyst*):TI,AB,KY

#18 (supernatant adj5 endometri*):TI,AB,KY

#19 (supernatant adj5 uter*):TI,AB,KY

#20 (endometri* adj5 inject*):TI,AB,KY

#21 (flush* adj5 endometri*):TI,AB,KY

#22 (flush* adj5 uter*):TI,AB,KY

#23 (culture* adj5 uter*):TI,AB,KY

#24 (culture* adj5 endometri*):TI,AB,KY

#25 (inject* adj5 uter*):TI,AB,KY

#26 (stimulat* adj2 endomet*):TI,AB,KY

#27 (culture* adj5 supernatant*):TI,AB,KY

#28 (flush* adj5 supernatant*):TI,AB,KY

#29 (transfer* adj5 supernatant):TI,AB,KY

#30 (inject* adj5 supernatant):TI,AB,KY

#31 (intrauter* adj5 inject*):TI,AB,KY

#32 (intrauter* adj5 flush*):TI,AB,KY

#33 (intrauter* adj5 supernatant*):TI,AB,KY

#34 (intrauter* adj5 culture):TI,AB,KY

#35 (instillation adj5 culture):TI,AB,KY

#36 (instillation adj5 uter*):TI,AB,KY

#37 MESH DESCRIPTOR Embryo Culture Techniques EXPLODE ALL TREES

#38 #14 OR #15 OR #16 OR #17 OR #18 OR #19 OR #20 OR #21 OR #22 OR #23 OR #24 OR #25 OR #26 OR #27 OR #28 OR #29 OR #30 OR #31 OR #32 OR #33 OR #34 OR #35 OR #36 OR #37

#39 #13 AND #38

Appendix 3. MEDLINE search strategy

From inception to present

Ovid platform

1 exp embryo transfer/ or exp fertilization in vitro/ or exp sperm injections, intracytoplasmic/
2 embryo transfer$.tw.
3 vitro fertili?ation.tw.
4 ivf‐et.tw.
5 icsi.tw.
6 intracytoplasmic sperm injection$.tw.
7 (blastocyst adj2 transfer$).tw.
8 ivf.tw.
9 or/1‐8
10 (inject* adj5 culture*).tw.
11 (inject* adj5 medi*).tw.
12 (supernatant adj5 embryo*).tw.
13 (supernatant adj5 blastocyst*).tw.
14 (supernatant adj5 endometri*).tw.
15 (supernatant adj5 uter*).tw.
16 (endometri* adj5 inject*).tw.
17 (flush* adj5 endometri*).tw.
18 (flush* adj5 uter*).tw.
19 (culture* adj5 uter*).tw.
20 (culture* adj5 endometri*).tw.
21 (inject* adj5 uter*).tw.
22 exp Embryo Culture Techniques/
23 (stimulat* adj2 endomet*).tw.
24 (culture* adj5 supernatant*).tw.
25 (flush* adj5 supernatant*).tw.
26 (transfer* adj5 supernatant).tw.
27 (inject* adj5 supernatant).tw.
28 (intrauter* adj5 inject*).tw.
29 (intrauter* adj5 flush*).tw.
30 (intrauter* adj5 supernatant*).tw.
31 (intrauter* adj5 culture).tw.
32 (instillation adj5 culture).tw.
33 (instillation adj5 uter*).tw.
34 or/10‐33
35 randomized controlled trial.pt.
36 controlled clinical trial.pt.
37 randomized.ab.
38 randomised.ab.
39 placebo.tw.
40 clinical trials as topic.sh.
41 randomly.ab.
42 trial.ti.
43 (crossover or cross‐over or cross over).tw.
44 or/35‐43
45 exp animals/ not humans.sh.
46 44 not 45
47 9 and 34 and 46

Appendix 4. Embase search strategy

From inception to present

Ovid platform

1 exp embryo transfer/ or exp fertilization in vitro/ or exp intracytoplasmic sperm injection/
2 embryo$ transfer$.tw.
3 in vitro fertili?ation.tw.
4 icsi.tw.
5 intracytoplasmic sperm injection$.tw.
6 (blastocyst adj2 transfer$).tw.
7 ivf.tw.
8 assisted reproduct$.tw.
9 ovulation induc$.tw.
10 superovulat$.tw.
11 COH.tw.
12 infertil$.tw.
13 subfertil$.tw.
14 (ovari$ adj2 induction).tw.
15 exp infertility therapy/
16 exp ovulation induction/
17 exp ovary hyperstimulation/
18 (ovar$ adj2 hyperstimulation).tw.
19 (ovar$ adj2 stimulat$).tw.
20 or/1‐19
21 exp embryo culture/ and (uter* or endometri*).tw.
22 (inject* adj5 culture*).tw.
23 (inject* adj5 medi*).tw.
24 (supernatant adj5 embryo*).tw.
25 (supernatant adj5 blastocyst*).tw.
26 (supernatant adj5 endometri*).tw.
27 (supernatant adj5 uter*).tw.
28 (endometri* adj5 inject*).tw.
29 (flush* adj5 endometri*).tw.
30 (flush* adj5 uter*).tw.
31 (culture* adj5 uter*).tw.
32 (culture* adj5 endometri*).tw.
33 (inject* adj5 uter*).tw.
34 (stimulat* adj2 endomet*).tw.
35 (culture* adj5 supernatant*).tw.
36 (flush* adj5 supernatant*).tw.
37 (transfer* adj5 supernatant).tw.
38 (inject* adj5 supernatant).tw.
39 (intrauter* adj5 inject*).tw.
40 (intrauter* adj5 flush*).tw.
41 (intrauter* adj5 supernatant*).tw.
42 (intrauter* adj5 culture).tw.
43 (instillation adj5 culture).tw.
44 (instillation adj5 uter*).tw.
45 or/21‐44
46 20 and 45
47 Clinical Trial/
48 Randomized Controlled Trial/
49 exp randomization/
50 Single Blind Procedure/
51 Double Blind Procedure/
52 Crossover Procedure/
53 Placebo/
54 Randomi?ed controlled trial$.tw.
55 Rct.tw.
56 random allocation.tw.
57 randomly.tw.
58 randomly allocated.tw.
59 allocated randomly.tw.
60 (allocated adj2 random).tw.
61 Single blind$.tw.
62 Double blind$.tw.
63 ((treble or triple) adj blind$).tw.
64 placebo$.tw.
65 prospective study/
66 or/47‐65
67 case study/
68 case report.tw.
69 abstract report/ or letter/
70 or/67‐69
71 66 not 70
72 (exp animal/ or animal.hw. or nonhuman/) not (exp human/ or human cell/ or (human or humans).ti.)
73 71 not 72
74 46 and 73

Appendix 5. CINAHL Plus search strategy

From inception to present

EBSCO platform

Appendix 6. World Health Organization International Trials Registry Platform and ClinicalTrials.gov registry

From inception to present

Keywords or Title CONTAINS

"IVF" or "ART" and "endometrial injection" or "embryo culture supernatant"